Commiphora – Myrrh (Myrrha, gummi-resina)
|Latin name of the genus:||Commiphora|
|Latin name of herbal substance:||Myrrha|
|Botanical name of plant:||Myrrh|
|English common name of herbal substance:||Gummi-resina|
Latin name of the genus: Commiphora
Latin name of herbal substance: Myrrha, gummi-resina
Botanical name of plant: Commiphora molmol Engler
English common name of herbal substance: Myrrh
- 1. Introduction
- 2. Historical data on medicinal use
- 3. Non-Clinical Data
- 3.1. Overview of available pharmacological data regarding the herbal substance(s), herbal preparation(s) and relevant constituents thereof
- 3.2. Overview of available pharmacokinetic data regarding the herbal substance(s), herbal preparation(s) and relevant constituents thereof
- 3.3. Overview of available toxicological data regarding the herbal substance(s)/herbal preparation(s) and constituents thereof
- 3.4. Overall conclusions on non-clinical data
- 4. Clinical Data
- 4.1. Clinical Pharmacology
- 4.1.1. Overview of pharmacodynamic data regarding the herbal substance(s)/preparation(s) including data on relevant constituents
- 4.1.2. Overview of pharmacokinetic data regarding the herbal substance(s)/preparation(s) including data on relevant constituents
- 4.2. Clinical Efficacy
- 4.2.1. Dose response studies
- 4.2.2. Clinical studies (case studies and clinical trials)
- 4.2.3. Clinical studies in special populations (e.g. elderly and children)
- 4.3. Overall conclusions on clinical pharmacology and efficacy
- 5. Clinical Safety/Pharmacovigilance
- 6. Overall conclusions
1.1. Description of the herbal substance(s), herbal preparation(s) or combinations thereof
Gumresin, hardened in air, obtained by incision or produced by spontaneous exudation from the stem and branches of Commiphora molmol Engler and/or other species of Commiphora, complying with the monograph of the European Pharmacopoeia (01/2008:1349).
Commiphora myrrha (Nees) Engler is a synonym of Commiphora molmol Engler. Other species which may be acceptable as sources of myrrh are Commiphora abyssinica (Berg) Engler and Commiphora schimperi (Berg) Engler (ESCOP 2003).
Myrrh tincture (1:5; extraction solvent: ethanol 90% V/V), Ph. Eur. monograph (01/2008:1877).
Combinations of herbal substance(s) and/or herbal preparation(s) including a description of vitamin(s) and/or mineral(s) as ingredients of traditional combination herbal medicinal products assessed, where applicable.
Combination products can be found in the EU: see section 1.2.
1.2. Information about products on the market in the Member States
According to the information provided by the National Competent Authorities:
Austria: Myrrh is present in combination products (as ethanolic extracts) used as bitters for stomach problems. Myrrh tincture (reported as magistral/officinal formula) is used in phytotherapy for inflammations of the gingiva.
Czech Republic: No single active ingredient products available, but several combination products („Schwedenbitter”) containing myrrh, used as adjuvants in mild gastrointestinal complaints, are available.
Denmark: Some MAs (from 1980) containing myrrh tincture, either alone or in combination products. Used as local astringent and anaesthetic in the mouth.
Estonia: One combination product registered.
Germany: Myrrh tincture (1:5; extraction solvent: ethanol 90% V/V) and a dry extract
Slovenia: No single active ingredient products available, but several combination products („Schwedenbitter”) containing myrrh, used as adjuvants in mild gastrointestinal complaints, are available.
United Kingdom: Myrrh tincture (British Pharmaceutical Codex) is available as a product first approved in 1972. The indications are: 1) Ulcers in the mouth and pharynx; 5 ml directly applied as required or
diluted with water to 20 ml and used as a gargle as required – 2) Flatulence;
Regulatory status overview
MA: Marketing Authorisation TRAD: Traditional Use Registration
Other TRAD: Other national Traditional systems of registration Other: If known, it should be specified or otherwise add ’Not Known’
This regulatory overview is not legally binding and does not necessarily reflect the legal status of the products in the MSs concerned.
1.3. Search and assessment methodology
PubMed was searched on 27 January 2010 using the terms [myrrh OR commiphora molmol OR commiphora mol mol OR commiphora myrrha]. One hundred twenty two references were retrieved. The abstracts were screened manually and all articles deemed relevant were assessed and included in the list of references.
2. Historical data on medicinal use
Myrrh appears to be one of the oldest medicines. Its use was recorded in the recipes from ancient Rome and in the texts of Hippocrates. Myrrh is also mentioned in both the Bible and the Koran (Madaus 1938).
2.1. Information on period of medicinal use in the Community
Myrrh has been used within the European Union for more than 30 years (Madaus 1938, Moritz 1967, Braun 1968). The herbal preparation mainly used seems to be the tincture (Todd 1967, Bradley 1992, Blumenthal 2000 (Commission E monograph dated 1987, BAnz Nr 193. 15.10.1987), ESCOP 2003, Barnes 2007, Ph. Eur. 2008).
2.2. Information on traditional/current indications and specified substances/preparations
The current use of myrrh in the form of tincture (1:5, extraction solvent ethanol 90% V/V) for oromucosal treatment of minor ulcers and inflammation in the mouth (stomatitis and gingivitis) is well- documented in recent handbooks (e.g. Bradley 1992, Blumenthal 2000, ESCOP 2003, Barnes 2007). The use of undiluted myrrh tincture to dab the affected areas in the mouth is also
Another current use of myrrh tincture is the topical application to minor wounds, abrasions, furuncles and skin inflammations (Bradley 1992, Hänsel 1992, ESCOP 2003, Barnes 2007).
Historically, myrrh (tincture) has had a medicinal use to relieve various gastrointestinal disorders, such as indigestion and intestinal infections (Madaus 1938, Todd 1967, Hänsel 1992, Bradley 1992, Information from UK), but this use seems to have declined (Hänsel 1992, Bradley 1992, ESCOP 2003, Barnes 2007). The oral use of myrrh tincture in combination products is still in practice within the Member States (Information from e.g. Austria, Czech Republic, Denmark, Estonia, Germany, Slovenia, UK).
2.3. Specified strength/posology/route of administration/duration of use for relevant preparations and indications
Recommended posology for oromucosal use of myrrh tincture (1 g = 65 drops; 0.8194 g/ml; 0.02 ml/drop) as a gargle:
5 ml of tincture in a glass of water 3 times daily (Bradley 1992).
5 ml of tincture in 15 ml of water (Information from UK).
Based on this information, the following posology appears well founded in European tradition:
Recommended posology for direct oromucosal application of myrrh tincture:
Apply the undiluted tincture to the affected areas on the gums or the mucous membranes of the mouth. Dab with a brush or swab,
Dab the affected areas
Apply undiluted tincture directly as required (information from UK).
Recommended posology for topical use of myrrh tincture on the skin:
Apply undiluted tincture (Bradley 1992).
For use on skin, dab
For skin, diluted or undiluted (Barnes 2007).
3.1. Overview of available pharmacological data regarding the herbal substance(s), herbal preparation(s) and relevant constituents thereof
Volatile oil, resin and gum. The main constituents of the volatile oil are
A review of myrrh, including products from other Commiphora species than the ones accepted as sources of myrrh, is published by El Ashry (2003).
Six aromatic sesquiterpenes were isolated from Commiphora myrrha Engler. One was a new furano- sesquiterpene and one was identified as a new natural aroma previously not found in the genus Commiphora. The others were isolated for the first time from Commiphora myrrha Engler (Zhu 2003). The sesquiterpenes
The octanordammaranes mansumbinone and
A review of the constituents found in myrrh and frankincense has been published by Shen (2008). The watersoluble gum fraction of myrrh (40% w/w) has been found to comprise a mixture of proteoglycans with dominating amounts of uronic acid rich polymers (Wiendl 1995).
Pharmacological effects relevant to traditional use:
Antibacterial and antifungal effects
A fraction from an
Staphylococcus aureus and Escherichia coli as well as antifungal effect against Candida albicans with minimum inhibitory concentrations (MIC) of
The crude chloroform extract of the
Assessor’s comments: Myrrh contains terpenes with fairly potent antibacterial effect against several bacteria including the most common wound pathogen S. aureus. The antibacterial effect has been shown in vitro and the mechanism of action is not known.
Local anaesthetic activity
A fraction from myrrh containing a mixture of
Assessor’s comments: Myrrh contains terpenes exhibiting local anaesthetic activity in animal experiments.
An ethanol extract of the
In rats, a petroleum ether extract (25:1) of the
Assessor’s comments: The oral doses required for
In mice, a suspension of ground myrrh in saline at an oral dose of 1 g/kg bw significantly (p<0.01) increased the latency of pain reaction (paw licking) when placed on a 52°C metal plate. The sesquiterpenes
Administered orally to mice, an ethanol extract of myrrh had a significant and
Assessor’s comments: In high doses, orally administered myrrh and isolated terpenes have an analgesic effect in animal experiments. The effect can be blocked with naloxone, which indicates an interaction with brain opioid mechanisms.
Other reported pharmacological effects:
After oral administration to hyperetic mice of either an ethanol or a petroleum ether extract of myrrh (25:1) at a dose of 500 mg/kg bw, a significant antipyretic effect (p<0.001) was demonstrated (Mohsin 1989, Tariq 1985).
Stimulation of phagocytosis
Mice inoculated with Escherichia coli were treated i.p. with either a dried ethanol extract or the unsaponifiable fraction of myrrh, as solutions in aqueous ethanol (10% V/V) at 50 mg/kg bw (1 mg per
20 g animal). Both treatments stimulated phagocytosis in over 80% of the mice compared to controls (Delaveau 1980).
Oral administration of myrrh to rats at 250, 500 and 1000 mg/kg bw provided significant and dose- dependent protection to the gastric mucosa against the ulcerogenic effects of various necrotising agents: 80% ethanol, 25% sodium chloride, 0.2 M sodium hydroxide, indometacin 30 mg/kg and combined ethanol
Protective effect against liver oxidative damage and genotoxicity
The effect of lead acetate in the diet (0.5% w/w) on reduced glutathione (GSH), activity on phase II metabolising enzyme glutathione
Antitumour and cytotoxic effects
The anticarcinogenic potential of Commiphora molmol (oleoresin) was studied in Ehrlich solid tumour- bearing mice. Treatment with C. molmol (250 and 500 mg/kg bw/day) was found to be cytotoxic in Ehrlich solid tumour cells. The
Oral doses of 125, 250 and 500 mg/kg bw of an aqueous suspension of myrrh given to mice for 7 days had no effect on the incidence of micronucleated polychromatic erythrocytes (PCE) in the bone marrow but in comparison to the control groups, there was a statistically significant decrease in the PCE/NCE ratio (NCE = normochromatic erythrocytes), indicating the cytotoxic potential of myrrh, which was found to be comparable to that of cyclophosphamide. The levels of DNA and protein in hepatic cells were not affected by treatment with myrrh but there was a significant decrease in their RNA content, comparable to that caused by cyclophosphamide. In Ehrlich ascites carcinoma (EAC)
Effects in gingival cells
Through their production of cytokines such as interleukin
0.001% MO decreased epithelial cell viability
The effect of MO on
Intragastric treatment of normal and diabetic rats with a 5% w/v aqueous extract of myrrh (extracted with boiling water then filtered) daily for one week at 10 ml/kg bw lowered fasting blood glucose levels in both groups and, in the oral glucose tolerance test, significantly increased glucose tolerance in both normal (p<0.02) and diabetic animals (p<0.05)
Oral administration of two fractions
Powdered myrrh (C. molmol), administered orally at 100 mg/kg bw, provided 86% (p<0.05) protection against
In several experimental studies in mice, the antischistosomal activity of a purified extract of myrrh (Mirazid®) has been investigated. Some studies have concluded that the efficacy is promising, whereas others have been unable to confirm this result (Hassan 2003, Massoud 2004a, Massoud 2004b, Massoud 2005, Hamed 2005, Botros 2004).
Assessor’s overall conclusions on pharmacology
Myrrh is a
A number of pharmacodynamic effects in animal experiments have been described for the resin as well as for isolated terpenes. Of relevance for the traditional indications gingivitis, stomatitis, wounds and furuncles are the reported antibacterial, local anaesthetic and
to the affected areas in the mouth or on the skin, so there is a reasonable plausibility that the experimentally observed effects may be clinically relevant.
In Egypt, myrrh is used for treatment of bilharzia and several Egyptian investigations have demonstrated antischistosomal activity of oral preparations of the herbal substance. This activity could, however, not be confirmed by other investigators. The potential antischistosomal activity of myrrh remains controversial.
3.2. Overview of available pharmacokinetic data regarding the herbal substance(s), herbal preparation(s) and relevant constituents thereof
3.3. Overview of available toxicological data regarding the herbal substance(s)/herbal preparation(s) and constituents thereof
Single dose toxicity
In mice, no visible signs of toxicity and no mortality were observed at oral doses of resin ≤ 3 g/kg bw (Rao 2001).
In rats, the acute oral LD50 value of myrrh oil is reported as 1.65 g/kg bw
Myrrh resin was administered to mice via the drinking water at a dose of 100 mg/kg bw/day during 3 months. No toxic symptoms were observed. The weight gain in the myrrh group was significantly (p<0.05) higher than in the control group. After treatment the average weight of the vital organs and conditions of the viscera were normal and comparable to that of the control animals. The weight of
testes, caudae epididymides and seminal vesicles was significantly (p<0.05) higher in the myrrh group as compared to the control. Biochemical studies showed no significant differences as compared to the control. Haematological studies revealed a significant (p<0.05) rise of RBC and haemoglobin levels in the myrrh group as compared to the control (Rao 2001).
Myrrh resin given daily to 6
Oral administration of a fresh aqueous suspension of myrrh to mice for 7 days at
Myrrh reduced the genotoxicity produced by lead acetate in mice
In rats, a preparation of myrrh (Mirazid®) caused a
Reproductive and developmental toxicity
No irritation, sensitisation (in 21 volunteers) or phototoxicity was found for myrrh oil (Opdyke 1976).
In tests of myrrh in the local lymph node assay (LLNA) in mice, a
Assessor’s overall conclusions on toxicology
Limited genotoxicity tests (micronucleus test in mice) have been performed, which indicated no genotoxic potential. Due to the antibacterial effect of myrrh, no results from Ames’ test are to be expected.
There are no reports on reproductive and developmental toxicity.
There are some reports in the literature about tests of the local tolerance of myrrh. An old report from 1976 in human volunteers indicated no irritating or sensitising effects, whereas a very recent study in the LLNA indicated a weak sensitising potential. The LLNA is a validated OECD1 test, and attention should be given to the sensitising potential of myrrh.
The analgesic effect following high doses of myrrh and the isolated terpenes
3.4. Overall conclusions on
Antibacterial, local anaesthetic and
There is also a historical medicinal use of myrrh and myrrh tincture for gastrointestinal disorders. This use possibly has some support by the experimental data on opioid and cytoprotective effects of myrrh. The oral use of myrrh (tincture) as single active ingredient seems to have declined during the last decades (Barnes 2007), and is not included in recent handbooks like Commission E (Blumenthal 2000) and ESCOP (2003).
4. Clinical Data
4.1. Clinical Pharmacology
No information available.
4.1.1. Overview of pharmacodynamic data regarding the herbal substance(s)/preparation(s) including data on relevant constituents
No information available.
1 Organisation for Economic
4.1.2. Overview of pharmacokinetic data regarding the herbal substance(s)/preparation(s) including data on relevant constituents
No information available.
Assessor’s overall conclusions on clinical pharmacology: No information on clinical pharmacology is available.
4.2. Clinical Efficacy
4.2.1. Dose response studies
No information available.
4.2.2. Clinical studies (case studies and clinical trials)
No clinical studies on the efficacy related to the traditional indications are available.
A commercial extract of myrrh – Mirazid®, marketed for treatment of schistosomiasis, has been subject to several clinical studies in Egypt but – in analogy with the pharmacodynamic studies of this preparation (see above) – the results are controversial. Two open studies, comprising a total of
212 patients, reported an excellent cure rate (92%) of an oral dose of 10 mg/kg bw/day for 3 days (Sheir 2001, Soliman 2004).
This result could not be confirmed in two other open studies. In one of these (Barakat 2005), Mirazid® was given to 45 patients in a dose of 2 capsules/day for 3 days and the effect compared to a dose of 40 mg/kg of praziquantel given to 38 other patients. The cure rate obtained with Mirazid® was 15.6%, compared to praziquantel (73.7%). Also a second treatment with Mirazid® gave a very low cure rate. In the second study (Botros 2005), a total of 1131 patients (459 school children and 672 household members) were randomly assigned to two groups. One group was given 300 mg/day of Mirazid® for 3 consecutive days and the second received praziquantel at a single dose of 40 mg/kg bw. Mirazid® showed cure rates of 9.1% and 8.9% in Schistosoma
The effect of myrrh for treatment of the zoonotic disease fascioliasis, caused by Fasciola, a liver fluke that infects sheep, goats and cattle for which humans act as an accidental host, has been investigated. 7 patients who were passing Fasciola eggs in their stools were treated with a formulation consisting of 8 parts of resin and 3.5 parts of volatile oils, all extracted from myrrh. The dose was 12 mg/kg bw/day for 6 consecutive days in the morning on an empty stomach. Patients were followed for 3 months. The therapy proved to be effective, with pronounced improvement of general condition and amelioration of all symptoms and signs. A dramatic drop in the egg count was detected at the end of treatment. Eggs were no longer detectable in the faeces 3 weeks after treatment and after a
3 months. High eosinophilic counts, elevated liver enzymes and Fasciola antibody titers returned to nearly normal. No signs of toxicity or adverse effects were observed (Massoud 2001).
Good cure rates were reported in an open study on treatment of human fascioliasis (liver fluke) with Mirazid®
4.2.3. Clinical studies in special populations (e.g. elderly and children)
4.3. Overall conclusions on clinical pharmacology and efficacy
No studies of clinical efficacy related to the traditional indications are available.
There is no doubt that myrrh has a
The results on antischistosomal effect and effect on liver fluke infection are controversial and require further studies before any firm conclusions can be made. There are no signals that myrrh has had a systematic use in these indications within the European Union.
5. Clinical Safety/Pharmacovigilance
Myrrh is listed by the Council of Europe as a natural source of favouring and may be added to foodstuffs in small quantities (Barnes 2007).
Myrrh and the essential oil have been evaluated by the Committee of Experts on Cosmetic Products of the Council of Europe and have been classified into Category A “Plants and plant preparations that can be used in cosmetic products”. Myrrh and the essential oil have cosmetic uses in e.g. toothpastes, mouthwashes and in fragrances (Council of Europe 2001).
5.1. Overview of toxicological/safety data from clinical trials in humans
No information available.
5.2. Patient exposure
No information available.
5.3. Adverse events and serious adverse events and deaths
Allergic contact dermatitis caused by the Chinese orthopaedic solution tieh ta yao gin was demonstrated to be due to the content of myrrh and mastic (Lee 1993a, 1993b).
Two cases of contact dermatitis due to plasters containing myrrh for treatment of wrist tendonitis have been reported (Gallo 1999).
Strong allergy to myrrh solution and myrrh powder, resulting from application of the products for wound healing, is reported
5.4. Laboratory findings
No information available.
5.5. Safety in special populations and situations
Drug interactions: A case of interaction with warfarin has been reported. The anticoagulant effect of warfarin was reduced after the patient had taken an aqueous extract of boiled roots of Commiphora molmol as a treatment for bronchitis (Al Faraj 2005).
Assessor’s comment: This report on boiled aqueous root extract of C. molmol is of limited relevance for the use of myrrh resin (tincture).
5.6. Overall conclusions on clinical safety
Very little clinical safety information is available. Apparently, there is a widespread cosmetic use of myrr and the essential oil, and the Council of Europe has approved this use. Nevertheless, there are a few reports on allergic skin reactions in the literature, and this is mentioned in the monograph.
6. Overall conclusions
Sufficient data have not been retrieved from the literature to support any
Myrrh and its tincture have a very long history and tradition of medicinal use. The required 30 years of medicinal use (including 15 years within the European Union) must be considered fulfilled.
The main areas of traditional medicinal use of myrrh and myrrh tincture have been treatment of infections/inflammation and pain in the mouth, infected wounds and other skin infections, and as a remedy for various gastrointestinal disorders.
The oral use for gastrointestinal disorders appears to have declined very much in the last 30 years and does not seem to be common practice today according to several authoritative handbooks of recent dates. The uses that still exist today appear to be limited to myrrh tincture as an ingredient in multi- component recipes. It is not recommended to include myrrh tincture for oral use in the Community herbal monograph.
The oromucosal use of myrrh tincture for oral infections/inflammations is currently in practice. For safety reasons, it appears reasonable to limit the use in self medication to the indications minor ulcers and inflammation in the mouth, such as stomatitis and gingivitis. These conditions are normally expected to benefit from an improved oral hygiene and the experimentally shown antibacterial, local anaesthetic and
The cutaneous use of myrrh tincture for treatment of infected wounds and skin inflammations has a long tradition. Again, the experimentally shown antibacterial, local anaesthetic and
The risks involved in oromucosal and cutaneous use of myrrh tincture are generally estimated to be low. A few clinical case reports in the literature and results from tests in mice point to a possibility of allergic skin reactions. Myrrh is, however, acceptable for use in both food and cosmetic products according to evaluations made by the Council of Europe.
Overall, a monograph on myrrh (tincture) is recommended with the following therapeutic indications:
a)Traditional herbal medicinal product for treatment of minor ulcers and inflammation in the mouth (stomatitis and gingivitis).
b) Traditional herbal medicinal product for treatment of minor wounds and small boils (furuncles).
with the statement that ‘The product is a traditional herbal medicinal product for use in specified indications exclusively based upon
In the absence of adequate tests on genotoxicity, no Community list entry can be established.